Mouse EGF Protein

Catalog No.: RMP023 | Size: 1mg

Source: E.col

Accession: P01132

Description: Recombinant Mouse Epidermal Growth Factor is produced by our E.coli expression system and the target gene encoding Asn977-Arg1029 is expressed with a 6His tag at the C-terminus.

Known As: Pro-epidermal growth factor; Epidermal growth factor; EGF

Predicted Mol Mass: 7.2 KDa

Apparent Mol Mass: 9-14 KDa, reducing conditions

Endotoxin: < 1 EU/µg as determined by LAL test

Formulation: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0

Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the                                          reconstituted solution to minimize freeze-thaw cycles.

Shipping: The product is shipped at ambient temperature.Upon receipt, store it immediately at the    temperature listed below.

Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.

                  Reconstituted protein solution can be stored at 2-8°C for 2-7 days.

                   Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.

Background: EGF is a single-pass type I membrane protein,containing 8 LDL-receptor class B repeats and 9     EGF-like domains. EGF results in cellular proliferation, differentiation,and survival.EGF is a low-molecular-weight polypeptide first purified from the mouse submandibular gland, but since then found in many human tissues including submandibular gland, parotid gland. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. The biological effects of salivary EGF include healing of oral and gastroesophageal ulcers, inhibition of gastric acid secretion, stimulation of DNA synthesis as well as mucosal protection from intraluminal injurious factors such as gastric acid, bile acids, pepsin, and trypsin and to physical, chemical and bacterial agents. 

Purity-SDS-PAGE:

Great
r than 95% as determined by reducing SDS-PAGE.

Bioactivity-Cell Based Assay：

Measured by its ability to inhibits the proliferation of human epithelial A431 cells.The ED50 for this effect is 2-20 ng/ml (QC verified).

Human endometrial epithelial organoids were cultured with EGF (Cat#CH28) and R-spondin 1 (Cat#CX83). The organoids showed good morphology. Data were kindly provided by Novoprotein's customer. Thanks for her contribution.