Recombinant Mouse M-CSF
Catalog No.: RMP019 | Size: 1mg

Source: Human Cells
Accession: P07141
Description: Recombinant Mouse Macrophage Colony-Stimulating Factor 1 is produced by our Mammalian expression system and the target gene encoding Lys33-Glu262 is expressed.
Known As: Macrophage colony-stimulating factor 1; CSF-1; MCSF; Csf1; Csfm
Predicted Mol Mass: 26 KDa
Apparent Mol Mass:     37-80 KDa, reducing conditions
Endotoxin: < 0.01 EU/µg as determined by LAL test
Formulation: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4
Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
Shipping: The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
              Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
              Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Background: Macrophage colony-stimulating factor 1(M-csf)is a single-pass type I membrane protein . It is a hematopoietic growth factor that is involved in the proliferation,differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways,including stimulating increased phagocytic and chemotactic activity, and     increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor, MCSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism,fertility and pregnancy. 

Purity-SDS-PAGE:

Greater than 95% as determined by reducing SDS-PAGE.

Purity-SEC-HPLC:

Greater than 92% as determined by SEC-HPLC. (QC verified)

Bioactivity-Cell Based Assay:

Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells.The ED50 for this effect is 0.04-0.2 ng/ml. (QC verified)